The proteinase inhibitor phenylmethylsulfonyl fluoride protects xanthine transport inSchizosaccharomyces pombeagainst inactivation by ammonium ions
نویسندگان
چکیده
منابع مشابه
Inactivation studies of acetylcholinesterase with phenylmethylsulfonyl fluoride.
Acetylcholinesterase (AChE), a serine hydrolase, is potentially susceptible to inactivation by phenylmethylsulfonyl fluoride (PMSF) and benzenesulfonyl fluoride (BSF). Although BSF inhibits both mouse and Torpedo californica AChE, PMSF does not react measurably with the T. californica enzyme. To understand the residue changes responsible for the change in reactivity, we studied the inactivation...
متن کاملTransport of ammonium and methylammonium ions by Azotobacter vinelandii.
Ammonium and methylammonium are rapidly taken up by cultures of Azotobacter vinelandii respiring in the presence of succinate. The rate of methylamine uptake increased with external pH from 5.5 to 7.5 but increasing the pH further to 8.5 had little effect on activity, indicating that methylammonium cation rather than uncharged methylamine is the permeant species. The kinetics of methylammonium ...
متن کاملEffect of the enzyme inhibitor, phenylmethylsulfonyl fluoride, on the IOP profiles of topical anandamides.
PURPOSE Earlier studies have suggested that the intraocular pressure (IOP) effects of topical arachidonylethanolamide (AEA) are mediated through its fatty acid metabolite, rather than through AEA, per se. The purpose of this study was to investigate whether the topical anandamides AEA and arachidonyl propionitrileamide (APN) decrease IOP when their enzymatic degradation is prevented by phenylme...
متن کاملAcceleration of the Rate of Reaction of Dimethylcarbamyl Fluoride and Acetylcholinesterase by Substituted Ammonium Ions.
The inhibition of acetylcholinesterase and other hydrolytic enzymes by methanesulfonyl fluoride, other sulfonyl fluorides, and anhydrides of methanesulfonic acid has been studied by several workers (2-5). Certain alkyl ammonium ions greatly increase the rate of irreversible inhibition of acetylcholinesterase by methanesulfonyl fluoride (6). The inhibition arises from the formation of a specific...
متن کاملThe Oxidative Inactivation of Human a-1-Proteinase Inhibitor
We have previously shown that the reactive center of human a-1-proteinase inhibitor (a-l-PI) contains a methionine at position P1 (Johnson, D., and Travis, J. (1978) J. Bid. Chem 263,7142-7144). The importance of this residue has been tested by oxidizing the inhibitor with increasing concentrations of N-chlorosuccinimide (SucNCl). Under the conditions utilized a maximum of 2 of the 8 methionyl ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Letters
سال: 1980
ISSN: 0014-5793
DOI: 10.1016/0014-5793(80)81189-6